Liquor analysis internal standard method

Preparation of the sample to be tested: (internal standard generally refers to: n-butyl acetate), purchased as

5ml

Chromatographically pure n-butyl acetate.

Liquor internal standard preparation steps:

(

1

)

60%

Ethanol preparation: take

150ml

Anhydrous ethanol (usually chromatographically pure) is made up to volume with purified water

250ml

.

(

2

)

2%

Internal standard preparation: take

5ml

N-butyl acetate

60%

Condensate ethanol to

250ml

.

(

3

) with sample wine sample preparation: take

10ml

Sample to be tested plus

0.2ml

of

2%

The internal standard, at this time the internal standard concentration in the wine sample is:

35.28mg/100ml

.

use

10ul

Microsampler

1ul

The wine sample to be tested can be.

Note: With wine sample preparation: Take

10ml

Sample to be tested plus

0.2ml

of

2%

Internal standard, the wine sample at this time

The internal standard concentration is:

35.28mg/100ml*2==0.56 mg/100ml

.

The liquor mixed standard is calculated by the instrument to calculate the correction factor:

N2000

Chromatography workstation as an example)

Liquor analysis internal standard method operation steps:

1.

Advanced liquor mixed standard

1ul

Standard online workstation, sample records and save the name, for example, a white wine standard

A

.

(Note that the first needle is generally not used, it is best to use the second needle.)

2.

Processing in an offline workstation, open → liquor standard

A

→OK→No→Map→Integration Method→Area→Inside

Mark → drift set

400

→ Use → Spectral → If the spectrum baseline is not good, you need to manually process the baseline → component table → select all → enter the peak name according to the peak time → select the component table before the internal standard peak (white wine is n-butyl acetate peak) Press and hold the mouse, left button is not raised to the first row of the component table → double click on the internal standard peak, change to → calibration → standard content → fill in the component content according to the content table of the mixed label →

OK

→Add standard samples→Select liquor standard

A

→ OK → Correction → Output → Input method file name, for example: liquor calibration curve

A

→Save.

3.

In the online workstation when analyzing the liquor sample: Open → liquor calibration curve

A

→ Open → Method → ​​Component Table → The internal standard concentration of the internal standard is filled into the internal standard concentration of the wine sample → use.

4.

Entering the wine sample with the internal standard

1ul,

Collect data, stop, save, and click preview.

5.

If the peak is not identified, the retention time in the component table should be changed to the current time offline → Adopt → Output