How to measure plant color by spectrophotometer - Database & Sql Blog Articles

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  • It is well known that plant leaves are the main organs in which plants perform photosynthesis and produce nutrients. By absorbing carbon dioxide, it releases oxygen to humans (release site: stomata), provides food, shelters from wind and rain. Leaves can have a variety of shapes, sizes, colors, and textures. The leaves can be clustered together or scattered all over the place. The edges of the leaves can be smooth or jagged.

    Src="http://i.bosscdn.com/blog/nophoto.gif" /> Figure 1 Plant leaves</p></p></p> <p ><p > There are many different colors, and there are many reasons for the ever-changing color of plant leaves. Environment such as soil, temperature, light, etc., other such as pH, enzymes, leaf epidermal cells. Therefore, people hope to observe the color changes of plant leaves at different stages and environments. The color changes that the human eye can observe are still very limited, and some subtle changes require us to be able to digitize. Therefore, according to the demand, people have developed an instrument that can observe the color change of plants at any time and anywhere - the spectrophotometer. </p></p> <p ><p ><p ><img alt=
    Figure 2 Spectrophotometer

    Let's take the spectrophotometer as an example to show you the steps to test the color of plant leaves:
    Step 1: Prepare the sample of the plant leaf to be tested (the leaves that grow at different stages) (Figure 3)

    Src="http://i.bosscdn.com/blog/nophoto.gif" /> Figure 3 Blades growing at different stages to be tested</p></p></p> <p > <p>Step 2: After the CS-600 spectrophotometer is turned on, perform black and white calibration (Figure 4);</p></p> <p ><p ><p ><img alt=
    Figure 4 black and white calibration

    Step 3: Because the color of the blade is not uniform, this will make the measured value not accurate enough. Therefore, we have to take the average value of multiple measurements to make the data more accurate.
    First, enter the setting before the measurement, and set the average setting by “UP” and “DOWN” to change the number of measurements to 5 times. (The specific number of times can be selected according to their own reasons, it is recommended to be at least 3 times)

    Src="http://i.bosscdn.com/blog/nophoto.gif" /> Figure 5 Setting the average setting</p></p></p> <p ><p >Step 4 : After setting, press “Cancel” to exit, then place the sample, then press “Test” button, “Drop” sound to complete the measurement, check the test result;;</p></p> <p ><p > <p ><img alt=
    Figure 6 standard measurement

    Step 5: After the standard sample is measured, press the “Menu” button to enter the “Sample Measurement” interface under the standard sample. Align the sample with the test hole according to the standard measurement method and press the “Test” button. After the measurement, the "drip" sound is completed and the color difference measurement is completed, and the measurement result can be obtained.

    Src="http://i.bosscdn.com/blog/nophoto.gif" /> Figure 7 sample 2 measurement results <img alt=
    Figure 8 sample 3 measurement results

    Through the measurement results, the user can visually see the color values ​​of the leaves growing at different stages and analyze the growth of the plants. Compared with the traditional visual method, the spectrophotometer has the characteristics of strong objectivity, quick and easy operation and high repeatability. It can help users understand plant growth more quickly and efficiently.

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