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It is mentioned in the pre-treatment of the sample that the supernatant of the cell culture medium is discarded, and the cells are scraped and transferred to a centrifuge tube to add the reagent 5. I would like to consult, in the sample preparation, the density of the cells is about to be available, the result is credible~ Thank you
Reply:
It is generally recommended that the number of cells be controlled at
5×10
6
More than one is appropriate, and the number is too small, the measurement result may be low, and the trend is not obvious.
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