GST Tag Fusion Protein - Purification, Detection
Glutathione S-transferase (GST) is a protein containing 211 amino acids which is usually added to the end of the recombinant protein for purification or detection of the recombinant protein. Non-fusion proteins with histidine produce non-specific binding, which prevents affinity purification of the histidine tag. But the GST tag is different. Since GST has extremely high specificity for its substrate, glutathione, higher purity can be obtained.
GST Tag Protein Purification Tool
GSH agarose gel or magnetic beads for efficient purification of GST tags and GST fusion proteins
The PurKineTM GST-Tag Purification System is based on an innovative high-load matrix that ensures efficient purification of recombinant GST fusion proteins and GSH-binding proteins from lysates of E. coli and mammalian cell expression systems in a single step. This product line has been specially optimized to be the optimal solution for ensuring the stability, solubility and yield of purified proteins. PurKineTM GST-Tag Purified Fillers are also available in pre-packed columns and kits for added convenience. In addition, Abbkine also offers products in the form of agarose gel resin fillers and magnetic beads.
PurKineTM GST-Tag Glutathione Resin 4FF has higher elution efficiency and protein loading than G brand products.
Fig. GST recombinant protein purification results comparison: PurKineTMGST-Tag Glutathione Resin 4FF (Lane B), G brand products (Lane C).
Precautions:
GST tag (glutathione thiotransferase) has mild elution conditions, helps maintain protein functional activity, is suitable for pull-down detection, has a good linear dynamic range, but has a large molecular weight, which may affect protein function. And downstream experiments, if the protein is insoluble, it is difficult to purify by denaturation.
GST tag protein detection antibody
Detection of GST-tag fusion proteins using GST-specific antibodies
GST Tag antibody is widely used in WB experiments to analyze the purification effect of GST-tagged protein, and it is very intuitive to judge whether the recombinant protein has been degraded.
As the general agent of Abbkine China, Amy Jiejie will provide customers with the most cost-effective routine immunological reagents, such as various tag antibodies, internal reference antibodies, conventional primary antibodies and a full range of secondary antibodies, antibody detection substrates, protein purification products and Related kits, etc. At the same time, as a Chinese agent or regional agent of many international famous brands, Aimei can provide you with a series of laboratory solutions. If you are interested in the above products, please call Ai Meijie Technology Co., Ltd. for product information and complete experimental solutions.
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