As a rapidly emerging high technology in the field of life sciences at the end of the last century, biochips will have to occupy several floors of biological laboratories and scale them down to one square centimeter in size. This will allow the analysis process to be fully automatic and the analysis speed will be improved. Thousands of times increase.
In recent years, China's bio-chip industry has moved from technology research and product development to technology application and product sales. Biochips are widely used in human genetic research, medical diagnostics, new drug development, optimization of crops, environmental testing, and many other fields. . However, it is not a simple matter to scale industrialization of biochips.
On September 18th, at the “2011 Advanced Symposium on Gene Chip Technology and Innovative Drug Application†sponsored by the China Association of Pharmaceutical Education, Fu Zhonghua, a senior engineer at the Institute of Genetics and Development of the Chinese Academy of Sciences, demonstrated the technology of flying point biochip preparation for everyone. Due to the extensive attention of the industry, the amazing speed of the technology has accelerated the progress of research work, improved the efficiency of the transformation of scientific research results, and successfully realized the industrialization of the scale of biochips.
Striking sample speeds The development of biochips is closely related to the development of manufacturing technology R&D platforms. According to Fu Zhonghua, the flying jet spot preparation technology is the result of research and development by the British company Arraet. At present, Arraet has developed 57 application preparation platforms worldwide. China also successfully introduced this preparation platform in June of this year, and achieved very good results.
"Flying point technology is a breakthrough and innovation in the biochip platform. This latest preparation platform is replacing the trend of the same kind of product." Fu Zhonghua said with certainty, "Its amazing sampling speed makes it possible to replace a machine. The past three or more."
Taking the series “marathon†as an example, the spotting platform can place 100 slides at a time. Under the density requirement of more than 50,000 points per slide, the spotter can reach the speed of 8.3 slides per minute. Another series of products is the “Super Marathonâ€. The spotting platform has ten slides to prepare the automatic lifting stepping system, and ten slides can be placed at one time to achieve a total of 25 slides per minute.
Why is the speed of spotting at flying jets so amazing? Fu Zhonghua explained that the key lies in the exquisite design of the nozzle. According to reports, the spotting head has a total of 128 nozzle positions. According to the requirements of the spot array, 96 are actually selected as the sampling heads. There are 12 and 32 two sampling needles for sample injection. , can absorb 12 or 32 samples at a time, at the same time, a sampling needle can once aspirate 500 points, each point is 2 nanoliters.
Not only that, according to the software's control and the combination of precise stepper motors and excellent slides, the flying spot speed can reach 0.2m/s. Each point can not only make one or more drops in flight. Point, gradient sampling, the volume of each point can also be well controlled, for research and industrialization can find more optimized methods to achieve the purpose of sample preparation, while also solving the problem of leakage in the preparation. Breakthrough features are significant.
Another highlight of the spotting platform is its microplate reservoir and slide storage expansion capabilities. According to Fu Zhonghua, if there are more samples, the storage function of the microplate reservoir can keep more samples, and according to the actual Need to automatically change the position of the orifice plate for automatic sampling and spotting; and the slide storage expansion function allows the preparation platform to be stacked ten layers at a time, with 100 slides per layer, and 1000 points of mechanized slides can be mechanized. kind.
In order to improve the spotting environment, in addition to the low-temperature, humidifying system, the flying point preparation platform has also added an ultra-clean space control system, which still has very good purification conditions in a space where the operating speed is extremely fast. This is also similar to other similar types. What the product does not have.
"Because of this optimization and good preparation space, the platform is suitable for point proteins, DNA and other samples." Fu Zhonghua said, "especially for the spotting of protein samples, due to the need to maintain the activity of protein samples, so for the sample The speed requirements are high, and this preparation platform just meets the speed requirements for protein spots."
The control of pollution is also a major concern for the preparation of chips. The flight spray point preparation platform uses a negative pressure injection cleaning method. The buffer is cleaned from the inside and outside of the pipeline at the same time. 12 injections and spotting devices can be cleaned multiple times to ensure samples. Not contaminated.
"Because of these breakthrough capabilities, it fully meets and solves the need for large-scale production of biochips," said Fu Zhonghua.
Promoting the Industrialization Process The principle of chip production technology is not complicated. In terms of each technology used in production and design, China has practical capabilities. Since 2000, the state has invested large sums of money to support the systematic development of biochips. Beijing National Chip Engineering Center, Shanghai National Chip Engineering Center, Xi’an Micro-Inspection Engineering Center, Tianjin Biochip Co., Ltd., and Nanjing Bio have been established. Labs and other R&D institutions in the chip.
In recent years, with the introduction of talents and projects, China has emerged a number of new types of viable companies, such as Guangzhou Ribo Bio, Kunming Biotech, Shanghai Biopharm, Shanghai Yulong Bio, Fujian Longyan Jiujian Bio Chip Technology Institute etc. However, due to the limitations of various conditions, there are still technical defects in the process of industrialization of biochips in China, especially in industrialization and preparation technology.
Fu Zhonghua said that compared with foreign products, China still has gaps in the series of products, spotting functions, as well as the precision, repeatability, spotting speed, and industrialization of the products. The products must be researched, piloted, and scaled up. In the industrial chain, it is obvious that China has not yet solved the bottleneck problem of industrialized production.
"Researching and introducing advanced foreign technologies will accelerate and promote the industrialization of China's biochips." Fu Zhonghua said, "The difficulty in the development of biochips in China is how to achieve the integration and integration of related technologies, and this new technology platform will be introduced. One difficulty was overcome successfully."
Fu Zhonghua suggested that in the process of developing the biochip industry, in addition to introducing advanced foreign technology, China should establish a stable and professional service team to timely follow up and solve the service problems of biochip technology.
“Arraet's technical service model team is worthy of our reference.†Fu Zhonghua said, “Arraet's team of experts uses many years of accumulated experience to provide services for those who need help free of charge. Samples are provided to Arraet’s team of experts and they will provide the best Production technology solutions to provide the most complete technical report."
In recent years, China's bio-chip industry has moved from technology research and product development to technology application and product sales. Biochips are widely used in human genetic research, medical diagnostics, new drug development, optimization of crops, environmental testing, and many other fields. . However, it is not a simple matter to scale industrialization of biochips.
On September 18th, at the “2011 Advanced Symposium on Gene Chip Technology and Innovative Drug Application†sponsored by the China Association of Pharmaceutical Education, Fu Zhonghua, a senior engineer at the Institute of Genetics and Development of the Chinese Academy of Sciences, demonstrated the technology of flying point biochip preparation for everyone. Due to the extensive attention of the industry, the amazing speed of the technology has accelerated the progress of research work, improved the efficiency of the transformation of scientific research results, and successfully realized the industrialization of the scale of biochips.
Striking sample speeds The development of biochips is closely related to the development of manufacturing technology R&D platforms. According to Fu Zhonghua, the flying jet spot preparation technology is the result of research and development by the British company Arraet. At present, Arraet has developed 57 application preparation platforms worldwide. China also successfully introduced this preparation platform in June of this year, and achieved very good results.
"Flying point technology is a breakthrough and innovation in the biochip platform. This latest preparation platform is replacing the trend of the same kind of product." Fu Zhonghua said with certainty, "Its amazing sampling speed makes it possible to replace a machine. The past three or more."
Taking the series “marathon†as an example, the spotting platform can place 100 slides at a time. Under the density requirement of more than 50,000 points per slide, the spotter can reach the speed of 8.3 slides per minute. Another series of products is the “Super Marathonâ€. The spotting platform has ten slides to prepare the automatic lifting stepping system, and ten slides can be placed at one time to achieve a total of 25 slides per minute.
Why is the speed of spotting at flying jets so amazing? Fu Zhonghua explained that the key lies in the exquisite design of the nozzle. According to reports, the spotting head has a total of 128 nozzle positions. According to the requirements of the spot array, 96 are actually selected as the sampling heads. There are 12 and 32 two sampling needles for sample injection. , can absorb 12 or 32 samples at a time, at the same time, a sampling needle can once aspirate 500 points, each point is 2 nanoliters.
Not only that, according to the software's control and the combination of precise stepper motors and excellent slides, the flying spot speed can reach 0.2m/s. Each point can not only make one or more drops in flight. Point, gradient sampling, the volume of each point can also be well controlled, for research and industrialization can find more optimized methods to achieve the purpose of sample preparation, while also solving the problem of leakage in the preparation. Breakthrough features are significant.
Another highlight of the spotting platform is its microplate reservoir and slide storage expansion capabilities. According to Fu Zhonghua, if there are more samples, the storage function of the microplate reservoir can keep more samples, and according to the actual Need to automatically change the position of the orifice plate for automatic sampling and spotting; and the slide storage expansion function allows the preparation platform to be stacked ten layers at a time, with 100 slides per layer, and 1000 points of mechanized slides can be mechanized. kind.
In order to improve the spotting environment, in addition to the low-temperature, humidifying system, the flying point preparation platform has also added an ultra-clean space control system, which still has very good purification conditions in a space where the operating speed is extremely fast. This is also similar to other similar types. What the product does not have.
"Because of this optimization and good preparation space, the platform is suitable for point proteins, DNA and other samples." Fu Zhonghua said, "especially for the spotting of protein samples, due to the need to maintain the activity of protein samples, so for the sample The speed requirements are high, and this preparation platform just meets the speed requirements for protein spots."
The control of pollution is also a major concern for the preparation of chips. The flight spray point preparation platform uses a negative pressure injection cleaning method. The buffer is cleaned from the inside and outside of the pipeline at the same time. 12 injections and spotting devices can be cleaned multiple times to ensure samples. Not contaminated.
"Because of these breakthrough capabilities, it fully meets and solves the need for large-scale production of biochips," said Fu Zhonghua.
Promoting the Industrialization Process The principle of chip production technology is not complicated. In terms of each technology used in production and design, China has practical capabilities. Since 2000, the state has invested large sums of money to support the systematic development of biochips. Beijing National Chip Engineering Center, Shanghai National Chip Engineering Center, Xi’an Micro-Inspection Engineering Center, Tianjin Biochip Co., Ltd., and Nanjing Bio have been established. Labs and other R&D institutions in the chip.
In recent years, with the introduction of talents and projects, China has emerged a number of new types of viable companies, such as Guangzhou Ribo Bio, Kunming Biotech, Shanghai Biopharm, Shanghai Yulong Bio, Fujian Longyan Jiujian Bio Chip Technology Institute etc. However, due to the limitations of various conditions, there are still technical defects in the process of industrialization of biochips in China, especially in industrialization and preparation technology.
Fu Zhonghua said that compared with foreign products, China still has gaps in the series of products, spotting functions, as well as the precision, repeatability, spotting speed, and industrialization of the products. The products must be researched, piloted, and scaled up. In the industrial chain, it is obvious that China has not yet solved the bottleneck problem of industrialized production.
"Researching and introducing advanced foreign technologies will accelerate and promote the industrialization of China's biochips." Fu Zhonghua said, "The difficulty in the development of biochips in China is how to achieve the integration and integration of related technologies, and this new technology platform will be introduced. One difficulty was overcome successfully."
Fu Zhonghua suggested that in the process of developing the biochip industry, in addition to introducing advanced foreign technology, China should establish a stable and professional service team to timely follow up and solve the service problems of biochip technology.
“Arraet's technical service model team is worthy of our reference.†Fu Zhonghua said, “Arraet's team of experts uses many years of accumulated experience to provide services for those who need help free of charge. Samples are provided to Arraet’s team of experts and they will provide the best Production technology solutions to provide the most complete technical report."
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